Composite

Part:BBa_K3031017:Design

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-15)


Iron QS system + ORF81 antigen protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2402
    Illegal AgeI site found at 2301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1907
    Illegal BsaI.rc site found at 2522
    Illegal SapI site found at 2491


Design Notes

FUR box position. Promoters for LuxR


Source

Biobricks NCBI for antigen

References